**Human Transforming Growth Factor β2 (TGF-β2) ELISA Kit – High-Quality Domestic Supplier from Guangrui Bio**
**Purpose:**
This ELISA kit is designed for the quantitative detection of Human Transforming Growth Factor β2 (TGF-β2) in various biological samples, including serum, plasma, urine, cell culture supernatants, and tissue homogenates. It provides a reliable and sensitive method for measuring TGF-β2 levels, which is essential in research related to inflammation, fibrosis, and immune regulation.
**Experimental Principle:**
The kit employs the double-antibody sandwich ELISA technique. A purified anti-TGF-β2 monoclonal antibody is pre-coated onto a microplate to serve as the solid-phase capture antibody. After incubation with the sample, TGF-β2 binds to the immobilized antibody. An HRP-conjugated secondary antibody is then added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following thorough washing, TMB substrate is introduced, and the reaction is stopped with an acidic solution. The color intensity, measured at 450 nm, is directly proportional to the concentration of TGF-β2 in the sample. A standard curve is used to calculate the exact concentration of TGF-β2 in each specimen.
**Kit Components:**
- 48-well configuration: 1×48 enzyme-labeled plate, 2 sealing films, 1 standard, 1 standard diluent, 1 sample diluent, 1 developer A, 1 developer B, 1 stop solution, 1 concentrated wash solution (20×).
- 96-well configuration: 1×96 enzyme-labeled plate, 2 sealing films, 1 standard, 1 standard diluent, 1 sample diluent, 1 developer A, 1 developer B, 1 stop solution, 1 concentrated wash solution (30×).
**Storage Instructions:**
All components should be stored at 2–8°C. The standard is provided at 900 ng/L, and all reagents must be kept away from light and moisture.
**Sample Preparation and Handling:**
1. **Serum:** Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant and re-centrifuge if precipitate forms.
2. **Plasma:** Use EDTA or sodium citrate as anticoagulant. Mix for 10–20 minutes, then centrifuge. Store and re-centrifuge if necessary.
3. **Urine:** Collect in a sterile tube and centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant.
4. **Cell Culture Supernatant:** Centrifuge after collection. For intracellular components, lyse cells by freezing-thawing, then centrifuge again.
5. **Tissue Samples:** Weigh the tissue, add PBS (pH 7.4), homogenize, and centrifuge. Store the supernatant at 2–8°C or freeze for later use.
6. **General Notes:** Process samples immediately after collection. If not tested right away, store at -20°C, avoiding repeated freeze-thaw cycles. Avoid using samples containing NaN3, as it inhibits HRP activity.
**Notes:**
This kit is ideal for researchers working in immunology, oncology, and inflammatory disease studies. Proper handling and storage ensure accurate and reproducible results. Always follow the manufacturer’s instructions for optimal performance.
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