**Human Transforming Growth Factor β2 (TGF-β2) ELISA Kit – Guangrui Bio, a Trusted Supplier of High-Quality ELISA Kits**
**Purpose:**
This ELISA kit is designed for the quantitative detection of Human Transforming Growth Factor β2 (TGF-β2) in various biological samples such as serum, plasma, urine, cell culture supernatants, and tissue homogenates. It provides a reliable and sensitive method to measure TGF-β2 levels, which is essential in research related to inflammation, immune regulation, and fibrosis.
**Experimental Principle:**
The kit utilizes a sandwich ELISA technique to detect TGF-β2. A microplate is pre-coated with a specific monoclonal antibody against TGF-β2. After adding the sample, TGF-β2 binds to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then added, forming an antibody-antigen-enzyme complex. Following a wash step, the substrate TMB is introduced, leading to a color change that is proportional to the amount of TGF-β2 present. The reaction is stopped with an acidic solution, and the absorbance is measured at 450 nm using a microplate reader. The concentration of TGF-β2 in the sample is determined by comparing the OD value to a standard curve.
**Kit Composition (48-well & 96-well configurations):**
- Microtiter plate: 1×48 / 1×96
- Standard: 0.5 ml × 1 bottle (900 ng/L)
- Standard Diluent: 1.5 ml × 1 bottle
- Enzyme Conjugate: 3 ml × 1 bottle
- Sample Diluent: 3 ml × 1 bottle
- TMB Substrate: 3 ml × 1 bottle
- Stop Solution: 3 ml × 1 bottle
- Wash Buffer (20×): 20 ml × 20 times / 20 ml × 30 times
- Sealing Films: 2 pieces (48-well) / 2 pieces (96-well)
- Sealing Bag: 1 piece
**Storage Instructions:**
All components should be stored at 2–8°C. Ensure the kit is kept away from moisture and direct light. Do not freeze the reagents unless specified.
**Sample Preparation and Handling:**
1. **Serum:** Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully; if precipitate forms, re-centrifuge.
2. **Plasma:** Use EDTA or sodium citrate as anticoagulant. Mix for 10–20 minutes, then centrifuge similarly.
3. **Urine:** Collect in a sterile container and centrifuge at 2000–3000 rpm for 20 minutes.
4. **Cell Culture Supernatant:** Centrifuge at 2000–3000 rpm for 20 minutes. For intracellular components, lyse cells via repeated freezing and thawing before centrifugation.
5. **Tissue Specimens:** Weigh the tissue, add PBS (pH 7.4), homogenize, and centrifuge. Store the supernatant at 2–8°C or freeze for later use.
6. **General Notes:** Process samples as soon as possible after collection. If storage is needed, keep at -20°C and avoid repeated freeze-thaw cycles. Avoid using samples containing NaN3, as it may inhibit HRP activity.
**Note:** This ELISA kit is ideal for researchers studying cytokine dynamics, autoimmune diseases, and tissue remodeling. Always follow the manufacturer's instructions and validate results with appropriate controls.
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