**Mouse Calcitonin Gene-Related Peptide (CGRP) ELISA Kit Instruction Manual**
**ELISA Kit Specifications:**
This Mouse CGRP ELISA Kit is available in two configurations: 48-well or 96-well. It is designed for quantitative detection of CGRP in biological samples such as serum, plasma, or tissue homogenates.
**Standard Dilution:**
A standard solution is provided at a concentration of 2700 ng/L, with a volume of 0.5 mL per bottle. This should be used to generate a standard curve for accurate quantification.
**Enzyme Standard Reagent:**
For the 48-well configuration, 3 mL × 1 bottle; for the 96-well configuration, 6 mL × 1 bottle. Store at 2–8°C.
**Kit Components:**
- Sealing Film: 2 pieces (for 48-well) / 2 pieces (for 96-well)
- Standard: 0.5 mL × 1 bottle (2700 ng/L)
- Sample Diluent: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Developer A: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Chromogen B: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Stop Solution: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Concentrated Washing Solution: 20 mL × 20 times (for 48-well) / 20 mL × 30 times (for 96-well)
**Storage Conditions:**
All reagents should be stored at 2–8°C unless otherwise specified. The Chromogen B solution must be stored at -20°C to maintain stability.
**Usage Instructions:**
This kit is intended for research use only. To determine the concentration of CGRP in your samples:
1. Prepare the standard curve by plotting the concentration (on the x-axis) against the OD value (on the y-axis).
2. Determine the corresponding concentration from the standard curve based on the OD value of your sample.
3. Multiply by the dilution factor if applicable.
Alternatively, calculate the linear regression equation of the standard curve using the standard concentrations and their corresponding OD values. Plug in the OD value of your sample into the equation to find the actual concentration, then multiply by the dilution factor to get the final result.
**Notes:**
- Ensure all reagents are brought to room temperature before use.
- Avoid contamination during the procedure.
- Follow the manufacturer's instructions carefully to ensure accurate results.
**Important:** Do not use this kit beyond the expiration date indicated on the packaging.
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