The experimental principle of the kit is based on the double-antibody sandwich enzyme immunoassay method to quantify insulin (INS) in the sample. A microplate is first coated with a purified rat insulin-specific antibody, creating a solid-phase capture antibody. The sample containing insulin is then added to the wells, allowing the insulin to bind to the immobilized antibody. Following this, an HRP-conjugated insulin antibody is introduced, forming a complex of antibody-antigen-enzyme-labeled antibody. After thorough washing to remove unbound components, a TMB substrate solution is added. Under the catalytic action of HRP, TMB turns blue, and upon addition of an acidic stop solution, it changes to a yellow color. The intensity of the yellow color is directly proportional to the concentration of insulin in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the concentration of rat insulin in the sample is determined by comparing the OD value to a pre-established standard curve. This method ensures high specificity and sensitivity for accurate insulin quantification.
Skin care is at the interface between cosmetic and dermatology, traditional medical disciplines; each subject has some overlap. The Food, Drugs and Cosmetics Act defines cosmetics as products intended to cleanse or beautify (such as shampoos and lipsticks). While some products (eg, moisturizing products) can be used to diagnose, cure, relieve, treat or prevent disease or affect the structure or function of the body (eg, sunscreens and acne creams), a separate category exists for sunscreens and anti-dandruff washes Hair water is regulated in both categories.
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