Human gamma interferon ELISA kit Chinese manual

Experimental principle: This kit uses the double antibody sandwich method to determine the level of human γ-interferon in the specimen. The microporous plate was coated with purified human interferon-gamma antibody to prepare a solid phase antibody, and γ interferon was sequentially added to the microwell of the coated monoclonal antibody, and then combined with the HRP-labeled γ-interferon antibody to form an antibody-antigen- The enzyme-labeled antibody complex was thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the gamma interferon in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human gamma interferon in the sample was calculated from a standard curve.

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