German Nanion automatic patch clamp port-a-patch and patchliner experiment process video

[Background introduction]:

The NPC automatic patch clamp system of the German Nanion company uses the world's most advanced scientific-grade flat-chip chip electrode technology, replacing the traditional patch clamp glass electrode. It is currently widely used in basic research and pharmacology of various ion channels the study. The chip has a small round hole of 1-2μm in the middle, and its unique design provides conditions for the automation of the patch clamp and the simplification of the experimental process. The introduction of a negative pressure controller (an air pump that can provide positive and negative pressure) greatly improves the success rate of cell sealing and membrane rupture, thereby achieving high-efficiency, high-quality, and high-quality patch clamp experimental analysis .

[Video Introduction]:

The first half of the video shows the experimental process of port-a-patch from 0 to 33 seconds.

We drop the cells over the small hole in the middle of the chip. At this time, the cells naturally settle and fall onto the small hole, and under the action of the negative pressure controller, a gigabit seal is formed and the membrane is broken. At this time, a stimulating voltage is given to the cell to open the ion channel on the cell membrane, and various ions (red) enter the cell through the membrane. When the inhibitor (green) interacts with the ion channel, the ion channel is closed and observed on the computer software The current to the channel is suppressed.

The bottom half of the video shows the patchliner's experimental process.

Patchliner's multi-channel fully automatic patch clamp implements fully automated operation, enabling 8 cells to perform experiments simultaneously. The replacement of various solutions, the dropping of cells, and the process of sealing and breaking the membrane are all completed by a computer program-controlled mechanical arm.

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